Detergent-Free Total Protein Isolation Kit
Fast and simple detergent-free isolation of total proteins
For research use only and NOT intended for in vitro diagnostics.
Detergent-Free Total Protein Isolation Kit
Fast and simple detergent-free isolation of total proteins
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Features and Benefits
- Simple protocol that is free of detergents
- Fast processing time
- Process a variety of inputs
- Compatible with mass spectrometry and NMR spectroscopy
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization.
The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.
Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
Details
Supporting Data
Figure 1. Detergent-free Proteins Isolated from E. coli, Yeast, HeLa Cells and Kidney Tissue. Total proteins were isolated from 2 x 109 E. coli cells, 2 x 107 yeast cells, 1 million HeLa cells and 15 mg of Chinese hamster kidney tissue in duplicate using Norgen’s Detergent-Free Total Protein Isolation Kit. The total proteins were analyzed by running aliquots on 12% SDS-PAGE gels. Panel A corresponds to 10 µL of the 40 µL recovered proteins from E. coli., Panel B corresponds to 10 µL of the 40 µL recovered proteins from yeast, Panel C corresponds to 12 µL of the 60 µL recovered proteins from the HeLa cells, while Panel D corresponds to 2 µL of the 60 µL of recovered proteins from the kidney tissue. The proteins are intact and of the highest quality, and can be used in a number of different downstream applications.
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Kit Specifications
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| Maximum Amount Of Starting Material: Tissues Animal Cells Bacteria Yeast |
50 mg 1 x 107 cells 1 x 1010 cells 1 x 109 cells |
| Time to Process 12 Samples | Less than 10 minutes |
Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 30300 (50 preps) |
|---|---|
| Lysis Solution | 4 mL |
| Lysis Tubes | 25 |
| Filter Columns | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
Documentation
Citations
| Title | Small colony variants of Burkholderia pseudomallei: alteration of the virulence factors |
| Citation | Journal of Taibah University for Science 2023. |
| Authors | Vanitha Mariappan,Muttiah Barathan,Ahmad Khusairy Zulpa,Jamuna Vadivelu &Kumutha Malar Vellasamy |
| Title | Proteomics Analysis Revealed that Crosstalk between Helicobacter pylori and Streptococcus mitis May Enhance Bacterial Survival and Reduces Carcinogenesis |
| Citation | Frontiers in Microbiology 2016. |
| Authors | Khosravi, Y., Loke, M. F., Goh, K. L., & Vadivelu, J |
| Title | Multiphasic strain differentiation of atypical mycobacteria from elephant trunk wash |
| Citation | PeerJ 2015. |
| Authors | Chan, K. G., Loke, M. F., Ong, B. L., Wong, Y. L., Hong, K. W., Tan, K. H., ... & Ngeow, Y. F |