Features and Benefits

Citrus Exocortis Viroid (CEVd) is a member of the genus Pospiviroid within the family Pospiviroidae. CEVd is the causal agent of the exocortis disease characterized by a bark shelling or scaling disorder of trifoliate orange used as rootstock. The citrange hybrids and Rangpur lime, both used as rootstocks, are also sensitive and develop bark scaling symptoms and stunting. Viroids are small plant pathogens consisting of a naked single-stranded circular RNA molecule of 246–475 nt, that do not encode any proteins but are endowed with autonomous replication in their host plants. Norgen’s Citrus Exocortis Viroid RT-PCR Detection Kit is a rapid and sensitive CEVd detection tool which can be applicable for the early diagnosis of CEVd.

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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TaqMan PCR Kit - Figure 1. Example of TaqMan PCR Positive result. Both PCR signals above the baseline from FAM and HEX channel indicate the successful PCR.

TaqMan PCR Kit - Figure 2. Example of TaqMan PCR Negative result. No target DNA was detected in FAM channel but amplification signal from HEX indicates the successful PCR.

TaqMan PCR Kit - Figure 3. Example of TaqMan PCR inhibition result. No signal from both FAM and HEX channel was detected. It is suggested to repeat the sample preparation using recommended kit for DNA purification.

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Documentation

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