Name: Saliva DNA Collection and Preservation Devices
Brand: Norgen Biotek Corp.
SKU: RU49000
Price: 679.000000 USD
Availability: InStock

Features and Benefits

Reliable and cost-effective
Non-invasive, user-friendly sample collection and preservation in one convenient kit
Preserved DNA is stable for 2 years at ambient temperature
Compatible with any commercial DNA isolation methods including Norgen’s Saliva DNA Isolation Kits (Cat. RU45400)
Samples are non-infectious and can be handled and shipped safely
Shipping accessories can be purchased separately
High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis

Norgen's Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components:

Saliva Collection Funnel and Collection Tube
Collection Tube Cap
Norgen's Saliva DNA Preservative contained within a sealed squeezable ampoule

Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). Each of Norgen's Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.

Saliva DNA Preservative

Norgen's Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.

DNA Isolation from Preservative

Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour. Saliva DNA can now be isolated from the preserved saliva samples using any commercially available method, including Norgen’s Saliva DNA Isolation Kit (Cat. RU45400), Norgen’s Saliva DNA Isolation 96-Well Kit (Cat. RU35200) and Norgen’s Saliva DNA Isolation Kit (Magnetic Bead System) (Cat. RU55400, RU62900). Also, Norgen’s Saliva DNA Isolation Reagent Kit (up to 4 mL) (Cat. RU35720) allows for DNA isolation from the preserved saliva samples using an alcohol precipitation method. The purified DNA is of the highest quality and can be used in a number of downstream applications including PCR, NGS and microarray analysis.

Details

Supporting Data

Figure 1. Stability of DNA Preserved in Norgen's Saliva DNA Preservative at Room Temperature for over 2 Years. Saliva samples were collected from numerous donors and mixed, and then an equal volume of Norgen's Saliva DNA Preservative was added to the saliva. The preserved saliva was then stored at room temperature for up to 24 months. Saliva DNA was subsequently isolated at 4 months, 8 months, 16 months and 24 months from 0.5 mL of the saliva/preservative sample using Norgen's Saliva DNA Isolation Kit (Cat. RU45400). For visual analysis, 100 µL of the purified DNA was run on an agarose TAE gel. As it can be seen, there is no evidence of DNA degradation after the saliva samples are stored for 24 months at ambient temperatures in Norgen's Saliva DNA Preservative. Furthermore, the size of the DNA was maintained over 24 kb for the entire 24 month period. M: Norgen's UltraRanger 1 Kb DNA Ladder (Cat. 12100).

Figure 2. GAPDH Real Time PCR Amplification of Saliva DNA from Saliva Samples Stored at Room Temperature from Day 0 up to 24 Months. All isolated saliva DNA samples were successfully amplified without any sign of PCR inhibition and difference in Ct. Red: Day 0, Blue: 4 months, Purple: 8 months, Green: 16 months and Sky blue: 24 months. Black: No template control.

Figure 3. V3-V4 16s rRNA PCR amplification for Illumina MiSeq 16s rRNA library preparation. The saliva DNA was isolated using Norgen's Saliva DNA Isolation Kit (Cat. RU45400) from saliva that had been preserved for various periods of time up to 2 years at room temperature in Norgen’s Saliva DNA Preservative. The PCR result shows the clean PCR amplification at ~550 bp indicating the right target of V3-V4 16s rRNA , with no difference between day 0 and 24 months. M: FastRunner DNA Ladder (Cat. 12800).

Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Over 2 years at room temperature

Shelf Life and Handling

When stored at room temperature, unused Norgen Saliva Preservative Tubes are stable through to the collect-before date without any reduction in performance. Please see the device insert or tube label for collect-before date.
Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Kit Components	Cat. RU49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1

Individual Saliva DNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1

Documentation

FAQs

What are the volume and dimensions of each saliva tube?

Each saliva tube is a 5 mL tube with dimensions as follows: 65 mm (height) x 16.5 mm (diameter).

What is the material of the saliva collection tube?

Tube material is as follows:

Cap: Polyethylene
Tube: Polypropylene (PP)
Funnel: Polyphenylene ether Polystyrene (PPE-PS) thermoplastic

Citations

Title	At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale
Journal	Human Reproduction. 2022.
Authors	Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort

Title	Exposure to traffic-related air pollution and bacterial diversity in the lower respiratory tract of children
Journal	PLOS ONE. 2021
Authors	Niemeier-Walsh, C., Ryan, P. H., Meller, J., Ollberding, N. J., Adhikari, A., & Reponen, T.

Title	Exploring influences on food choice in a large population sample: the Italian Taste Project
Journal	Food Quality and Preference. 2017
Authors	E. Monteleone, S. Spinelli, C. Dinnella, I. Endrizzi, M. Laureati, E. Pagliarini, F. Sinesio, F. Gasperi, L. Torri, E. Aprea, L.I. Bailetti, A. Bendini, A. Braghieri, C. Cattaneo, D. Cliceri, N. Condelli, M.C. Cravero, A. Del Caro, R. Di Monaco, S. Drago, S. Favotto, R. Fusi, L. Galassi, T. Gallina Toschi, A. Garavaldi, P. Gasparini, E. Gatti, C. Masi, A. Mazzaglia, E. Moneta, E. Piasentier, M. Piochi, N. Pirastu, S. Predieri, A. Robino, F. Russo, F. Tesini

Title	Sugar-Sweetened Beverage Consumption Is Adversely Associated with Childhood Attention Deficit/Hyperactivity Disorder
Journal	International Journal of Environmental Research and Public Health. 2016.
Authors	Yu CJ, Du JC, Chiou HC, Feng CC, Chung MY, Yang W, Chen YS, Chien LC, Hwang B, Chen ML

Title	Detection of Novel Integrons in the Metagenome of Human Saliva
Journal	PLOS ONE. 2016.
Authors	Tansirichaiya S, Rahman MA, Antepowicz A, Mullany P, Roberts AP

Title	Increased risk of attention-deficit/hyperactivity disorder associated with exposure to organophosphate pesticide in Taiwanese children
Journal	Andrology. 2016.
Authors	Yu CJ, Du JC, Chiou HC, Chung MY, Yang W, Chen YS, Fuh MR, Chien LC, Hwang B, Chen ML

Title	Attention Deficit/Hyperactivity Disorder and Urinary Nonylphenol Levels: A Case-Control Study in Taiwanese Children
Journal	PLOS ONE. 2016.
Authors	Yu CJ, Du JC, Chiou HC, Yang SH, Liao KW, Yang W, Chung MY, Chien LC, Hwang B, Chen ML

Title	Functional polymorphisms in the P2X7 receptor gene are associated with stress fracture injury
Journal	Purinergic Signalling. 2016.
Authors	Varley I, Greeves JP, Sale C, Friedman E, Moran DS, Yanovich R, Wilson PJ, Gartland A, Hughes DC, Stellingwerff T, Ranson C, Fraser WD, Gallagher JA

Title	Selective familiarity deficits in otherwise cognitively intact aging individuals with genetic risk for Alzheimer's disease
Journal	Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring. 2015.
Authors	Dorothee Schoemaker, Judes Poirier, Sophia Escobar, Serge Gauthier, Jens Pruessner

Title	The dentin phosphoprotein repeat region and inherited defects of dentin
Journal	Molecular Genetics & Genomic Medicine. 2015.
Authors	Jie Yang, Kazuhiko Kawasaki, Moses Lee, Bryan M. Reid, Stephanie M. Nunez, Murim Choi, Figen Seymen, Mine Koruyucu, Yelda Kasimoglu, Ninna Estrella-Yuson, Brent P. J. Lin, James P. Simmer and Jan C.-C. Hu

Title	Taurodontism, variations in tooth number, and misshapened crowns in Wnt10a null mice and human kindreds.
Journal	Molecular Genetics & Genomic Medicine. 2014.
Authors	Jie Yang, Shih-Kai Wang, Murim Choi, Bryan M. Reid, Yuanyuan Hu, Yuan-Ling Lee, Curtis R. Herzog, Hera Kim-Berman, Moses Lee, Paul J. Benke, K. C. Kent Lloyd, James P. Simmer and Jan C.-C. Hu.

Title	Patterns of Admixture and Population Structure in Native Populations of Northwest North America.
Journal	PLOS Genetics. 2014.
Authors	Paul Verdu, Trevor J. Pemberton, Romain Laurent, Brian M. Kemp, Angelica Gonzalez-Oliver, Clara Gorodezky, Cris E. Hughes, Milena R. Shattuck,Barbara Petzelt, Joycelynn Mitchell, Harold Harry, Theresa William, Rosita Worl, Jerome S. Cybulski, Noah A. Rosenberg, Ripan S. Malhi.

Title	Association of premenstrual/menstrual symptoms with perinatal depression and a polymorphic repeat in the polyglutamine tract of the retinoic acid induced 1 gene
Journal	Journal of Affective Disorders. 2014.
Authors	Ene-Choo Tan, Hui-San Tan, Tze-Ern Chua, Theresa Lee, Jasmine Ng, Ying-Chia Ch’ng, Chih-Huei Choo, Helen Y Chen

Title	RANK/RANKL/OPG pathway: Genetic associations with stress fracture period prevalence in elite athletes.
Journal	The Bone Journal. 2014.
Authors	Ian Varley, David C. Hughes, Julie P. Greeves, Trent Stellingwerff, Craig Ranson, William D. Fraser, Craig Sale.

Title	Differences in the quantity of DNA found in the urine and saliva of smokers versus nonsmokers: implications for the timing of epigenetic events.
Journal	Epigenomics. 2012.
Authors	Simkin M, Abdalla M, El-Mogy M, Haj-Ahmad Y.

Saliva DNA Collection and Preservation Devices (50)