2X PCR Master Mix
Supporting Data
Figure 1. Successful Amplification of Different Sized Amplicons. Norgen’s 2X PCR Master Mix was used in a series of PCR reactions to amplify a number of different sized amplicons from the adenovirus E2 region. Lanes 1 and 2 correspond to a 189 bp product, Lanes 3 and 4 correspond to a 565 bp product, Lanes 5 and 6 correspond to a 1501 bp product, and Lanes 7 and 8 correspond to a 2202 bp product. Norgen’s 2X PCR Master Mix was able to amplify all the different sized products with a high sensitivity and yield. Lane M1 is Norgen’s PCR Sizer 100 bp DNA Ladder (Cat #11400) and Lane M2 is Norgen’s MidRanger 1kb DNA Ladder (Cat # 11700).
Figure 2. Highly Stable after Multiple Freeze-Thaw Cycles The stability of Norgen’s 2X PCR Master Mix was tested by subjected the mix to multiple freezing and thawing cycles, followed by using the Master Mix to amplify the 16s rDNA (1.5 kb) of Staphylococcus aureus gDNA. Norgen’s 2X PCR Master Mix showed robustness and outstanding stability from multiple freezing and thawing steps at -20ºC. M: FastRunner DNA Ladder (Cat#12800). Ten μL of each PCR reaction was loaded on a 1% TAE agarose gel.
Reagents Supplied - Cat # 28007
- 2X PCR Master Mix (1 Vial, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions
Storage Conditions and Product Stability
Norgen’s 2X Master Mix should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles (see Figure 2). When stored at the proper temperature this reagent is stable for at least 1 year.