Name: FFPE RNA/DNA Purification Plus Kit Dx
Brand: Norgen Biotek Corp.
SKU: Dx54300
Price: 655.000000 USD
Availability: InStock

Supporting Data

Figure 1. Superior Recovery of High Quality RNA and DNA from FFPE Spleen Tissues. Norgen's FFPE RNA/DNA Purification Plus Kit Dx isolates FFPE RNA and DNA that exceeds the yield of competitors. Total RNA and DNA was isolated from equal amount of hamster FFPE spleen sections (20 micron thickness) using Norgen's FFPE RNA /DNA Purification Plus Kit Dx and a leading competitor’s kits. Triplicate isolations were performed for each product. The top graphs demonstrate the mean yield of RNA (Panel A) and DNA (Panel B) according to NanoDrop measurement. The bottom graphs showed the mean 260:280 ratio and 260:230 ratio of RNA (Panel C) and DNA (Panel D) according to NanoDrop measurement. Norgen's kit consistently purified total RNA and DNA with a higher yield and higher quality than for those obtained using the market competitor's kits.

Figure 2. Recovery of High Quality DNA and True Total RNA including microRNA from Hamster Spleen. Genomic DNA isolated using Norgen's FFPE RNA/DNA Purification Plus Kit Dx is of high quality with effective qPCR amplification. Panel A showed that in a qPCR using primers against the 5S rRNA gene using equal amount of FFPE DNA as template, the DNA isolated with Norgen's kit showed better amplification (lower Ct value). Moreover, Norgen's FFPE RNA/DNA Purification Plus Kit isolates true total RNA including microRNA. With the same amount of RNA as template in RT-qPCR reactions for transcripts of miR-21 (Panel B for microRNA) and beta-Actin (Panel C for mRNA), RNA isolated by Norgen showed better amplification in beta-Actin and significantly better in miR-21 microRNA.

Kit Specifications
Maximum Column Binding Capacity (RNA)	35 μg
Maximum Column Binding Capacity (gDNA)	10 μg
Maximum Column Loading Volume	600 μL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material	4 sections <20 μM thick 10 mg of unsectioned block

Storage Conditions and Product Stability

The DNAse I and Proteinase K should be stored at -20°C upon arrival. All other solutions and kit components should be kept tightly sealed and stored at room temperature. All solutions and plastics can be used until the expiration date specified on their labels.

Component	Cat. 54300 (50 preps)
Digestion Buffer	2 x 20 mL
Binding Solution	2 x 20 mL
Enzyme Incubation Buffer A	6 mL
RNA Wash Solution	24 mL
DNA Wash Solution	38 mL
RNA Elution Solution	6 mL
DNA Elution Buffer	15 mL
Proteinase K	2 x 12 mg
DNase I	200 μL
RNA Purification Micro Columns	50
DNA Purification Micro Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	100
Product Insert	1

FFPE RNA/DNA Purification Plus Kit Dx