Figure 2. Resolution of DNA isolated from buffy coats using Norgen's Blood Genomic DNA Isolation Mini Kit. 15 μL of 200 μL elutions were run on 1X TAE 1.0% agarose gel. Marker= Norgen's UltraRanger DNA Ladder.

Figure 3. The difference in Ct values between DNA isolated using Norgen's Blood Genomic DNA Isolation Mini Kit (Norgen), Competitor A's DNA Blood Mini Kit (A), Competitor B's Blood Kit (B), and Competitor B's Dx Blood (B Dx), in a Taqman qPCR reaction. Different template volumes (1, 3, 6 & 9 μL) of each elution were used in a 20 μL qPCR reaction involving GAPDH primers.

Figure 4. The difference in yield isolated from different volumes of blood using Norgen's vs Qiagen's kit. 50 μL of each sample was diluted in 450 μL of nuclease-free water, and DNA concentrations were measured using the UltraSpec 2100 Pro (Fisher Scientific). Two elutions were performed for all samples.

Figure 5. High Yields of DNA Isolated from Whole Blood. DNA was isolated from two different 10mL whole blood samples using Norgen's Blood DNA Isolation Maxi Kit. Following isolation, 10 µL from each 1 mL elution was loaded on 1% TAE agarose gel. Lanes 1 and 3 correspond to the first elution, while Lanes 2 and 4 correspond to the second elution. Norgen's Blood DNA Isolation Maxi Kit demonstrated a high yield of intact DNA.

Figure 6. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 10 mL of whole human blood using Norgen's Blood DNA Isolation Maxi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 1mL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 7. High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood. DNA was isolated from 20, 50, 100 and 200 µL of whole blood using Norgen's Blood DNA Isolation Mini Kit and a leading competitor's kit. Following isolation, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Mini Kit demonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgen’s UltraRanger 1kb DNA Ladder.

Figure 8. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). 9 µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNA isolated using Norgen's kit. The black line is a no-template control.

Figure 9. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 20, 50, 100 and 200 µL of whole human blood using Norgen's Blood DNA Isolation Mini Kit (Blue) and a leading competitor's kit (Red). Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNA isolated using Norgen's kit.

Figure 10. High Yields of DNA Isolated from 2 mL of Whole Blood. DNA was isolated from 2 mL of whole blood using Norgen's Blood DNA Isolation Midi Kit. Following isolation, 20 µL from 300 µL first and second elutions was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Midi Kit demonstrated a high DNA yield and integrity. Lane L: Norgen's HighRanger 1kb DNA Ladder.

Figure 11. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 12. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 2 mL of whole human blood using Norgen's Blood DNA Isolation Midi Kit. Different input amounts (1, 3 & 6 µL) of the DNA from each of the 300 µL elutions was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene with a linear decrease in Ct value with the increased DNA template volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications.

Figure 13. High Yields of DNA Isolated from 200 µL of Whole Blood. DNA was isolated from 200 µL of whole blood using Norgen's Blood DNA Isolation 96-Well Kit. Following isolation of 12 samples, 15 µL from each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation 96-Well Kit demonstrated a good and consistent DNA yield and integrity. Lane M: Norgen's HighRanger 1kb DNA Ladder.

Figure 14. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNA was isolated from 200 µL of whole human blood using Norgen's Blood DNA Isolation 96-Well Kit. Five µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene from all the isolated 12 samples (blue). This indicates that the isolated DNA from all samples is of a high quality and can be used in sensitive downstream applications. The black line is a no-template control.

Figure 15. DNA Isolated from Blood Preserved in 3 Different Anticoagulants. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin: H, EDTA: E and Na Citrate: C) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. Norgen's Blood DNA Isolation Kit (Magnetic Bead System) showed an intact and comparable DNA profile to the column method from all three different anticoagulant mixed blood samples. Marker = Norgen's HighRanger DNA Ladder.

Figure 16. High Yield of Blood DNA. DNA was isolated from 200 µL of human whole blood samples preserved in three different anticoagulants (Heparin, EDTA and Na Citrate) using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The total DNA yield from all the elutions was compared, and it was found that the Magnetic Bead System isolated a similar DNA yield to the column method from all the different anticoagulants tested.

Figure 17. Detection of GAPDH from blood samples preserved in three different anticoagulants (Heparin: red, EDTA: green and Na Citrate: blue). DNA was isolated from 200 µL human whole blood samples preserved in three different anticoagulants using Norgen's Blood DNA Isolation Kit (Magnetic Bead System) and Norgen's Blood DNA Isolation Mini Kit (column format, Cat. 46300). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL (circle), 4 µL (triangle) and 8 µL (cross) (total PCR reaction volume was 20 µL). No PCR inhibition was observed up to 8  µL of PCR template, indicating the excellent quality of blood DNA isolated using Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) similar to the column method (Cat. 46300).

Figure 18. DNA Isolated from Blood Preserved in EDTA Anticoagulants. DNA was isolated from 200 µL of human whole blood samples using Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System). For evaluation, 10 µL from each 200 µL of elution were run on 1X TAE 1.2% agarose gel. Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System) showed an intact DNA profile. Marker = Norgen's HighRanger DNA Ladder (Cat. 11900).

Figure 19. Detection of GAPDH From Blood Samples Preserved in EDTA Anticoagulants. DNA was isolated from 200 µL human whole blood samples using Norgen's Blood DNA Isolation 96-Well Kit (Magnetic Bead System). The high quality of the purified DNA was confirmed by Real-time PCR using 2 µL of purified DNA in a total PCR reaction volume of 20 µL. No PCR inhibition was observed, indicating the excellent quality of blood DNA isolated using Norgen’s Blood DNA Isolation 96-Well Kit (Magnetic Bead System).