cf-DNA/cf-RNA Preservative Tubes
For research use only and NOT intended for in vitro diagnostics.
CE-IVD diagnostic version available here
cf-DNA/cf-RNA Preservative Tubes
SKU
63950
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Supporting Data
Kit Specifications
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Tube Size
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16 x 100 mm
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Tube Type | PET Tube |
Blood Draw Volume
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8.7 mL in 10 mL Tubes
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Preservative Volume |
1.3 mL
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Recovered Plasma Volume | Approximately 6-7 mL (not affected by shipping) |
Anticoagulant |
Proprietary
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Preservative |
Non formaldehyde - Proprietary preservation agent
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Length of cf-DNA Preservation |
Up to 30 days at room temperature (15-25°C)
Up to 8 days at 37°C |
Length of cf-RNA Preservation |
Up to 30 days at room temperature (15-25°C)
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Length of CTCs Preservation |
Up to 14 days at room temperature (15-25°C)
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Storage Conditions and Product Stability
- When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
- Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
- Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
- Cell-free DNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
- Cell-free DNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
- Cell-free RNA in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
- Circulating tumor cells (CTCs) in blood samples collected in Norgen's cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).
Documentation
cf-DNA/cf-RNA Preservative Tubes - Mixing Instructions
Isolation of White Blood Cells (WBCs) / Circulating Tumour Cells (CTCs) from cf-DNA/cf-RNA Preservative Tubes
Supp Protocol PI63950-RNA from WBCs and CTCs_0.pdf
Supplementary Protocol PI63950-2-DNA from WBCs and CTCs.pdf
Supplementary Protocol PI63950-2 RNA DNA Protein Purification from Leukocytes.pdf
Isolation of White Blood Cells (WBCs) / Circulating Tumour Cells (CTCs) from cf-DNA/cf-RNA Preservative Tubes
Supp Protocol PI63950-RNA from WBCs and CTCs_0.pdf
Supplementary Protocol PI63950-2-DNA from WBCs and CTCs.pdf
Supplementary Protocol PI63950-2 RNA DNA Protein Purification from Leukocytes.pdf
FAQs
pH of blood/ plasma preservative is 7.
This is expected at cold temperatures during shipping, and the tubes are good to use. Please refer to the product insert - Section A.
Yes, the EVs are also preserved for up to 7 days at room temperature in the sample collected using these tubes. Please contact our technical support team at support@norgenbiotek.com and ask for more information.
The blood should be mixed with the preservative immediately (within 1 minutes of collection) by gentle and complete inversion of the tube 5-10 times. Improper mixing, vortexing or vigorous mixing will harm the sample.
Yes, please follow the Supplementary protocols where this information is provided in detail.