microScript microRNA cDNA Synthesis Kit
For research use only and NOT intended for in vitro diagnostics.
microScript microRNA cDNA Synthesis Kit
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Supporting Data
Figure 1. High Sensitivity with Robust Amplification of microRNAs from Different Amounts of RNA Input using Norgen’s microScript microRNA cDNA Synthesis Kit. Norgen’s microScript microRNA cDNA Synthesis Kit was used in an RT-qPCR reaction to amplify the human miR-1246 and miR-21 from a dilution series of HeLa total RNA isolated using Norgen’s Total RNA Purification Kit. The PCR target was robustly amplified from a broad range of RNA inputs, from the 200 ng down to 2 pg.
Figure 2. Robust Reverse Transcription of All RNA Transcripts (microRNAs and mRNA). Norgen’s microScript microRNA cDNA Synthesis Kit could effectively reverse transcribe both microRNA and other RNA species such as mRNA and large RNAs, allowing the parallel study of microRNA and its targets. HeLa RNA (200 ng) was used as a template in a reverse transcription using the microScript microRNA cDNA Synthesis Kit. The resulting cDNA was used in three different qPCR reactions for three different species of RNA. The top three panels showed the successful PCR amplification curve for miR-21 (microRNA), 5S rRNA (RNA without poly-A tail) and S15 (large mRNA). The lower three panels showed that each of the three PCR amplification showed unique amplicon with unique melt curve characteristics.
Storage Conditions and Product Stability
This kit is stable for 1 year after the date of shipment .
| Component | Cat. 54415 (12 rxns) | Cat. 54410 (50 rxns) |
|---|---|---|
| microScript microRNA Enzyme Mix | 12 μL | 50 μL |
| 2x Reaction Mix | 120 μL | 500 μL |
| Universal PCR Reverse Primer | 60 μL | 250 μL |
| Nuclease-Free Water | 1.25 mL | 2 x 1.25 mL |
| Product Insert | 1 | 1 |